Centrifuge operation method

Centrifugation is the use of the centrifugal force generated by the high speed rotation of the centrifuge rotor to accelerate the settling velocity of the particles in the liquid and separate the sedimentation coefficient and the buoyancy density of the sample. Therefore, it is necessary to use a centrifuge to generate a strong centrifugal force in order to force these particles to overcome the diffusion and produce a settling motion. So how does the operation method of the centrifuge come? Let's take a look at it together.

1. When using various centrifuges, the centrifuge tube and its contents must be precisely balanced on the scale in advance. The difference in weight during the balance must not exceed the range specified in the specifications of each centrifuge. Each centrifuge has a different rotor. The respective allowable difference, absolutely can not be loaded with a single number of tubes in the rotor, when the rotor is only partially loaded, the tubes must be symmetrically placed in the rotor in order to evenly distribute the load around the rotor.

2. If you want to centrifuge at a temperature lower than room temperature. The rotor should be placed in the refrigerator or in the turret chamber of the centrifuge before use.

3, during the centrifugation process must not leave, should always observe the centrifuge on the instrument is working properly, if abnormal sound should immediately stop the inspection, timely troubleshooting.

4, each rotor has its maximum allowable speed and cumulative use of time limit, when using the rotor to consult the instructions, not too fast. Each rotor must have a usage file. The accumulated use time should be recorded. If it exceeds the maximum usage limit of the rotor, it must be used at a reduced speed.

5, when loading the solution, according to the specific operating instructions of various centrifuges, according to the nature and volume of the liquid to be centrifuged, select the appropriate centrifuge tube, and some centrifuge tubes without lid, the liquid can not be installed too much to prevent the centrifuge The spin-off results in unbalanced, rusted, or corroded rotor heads. Centrifugal tubes of preparative ultracentrifuges often require that the liquid must be filled to prevent the upper part of the plastic centrifuge tubes from sagging during centrifugation. After each use, the head must be carefully inspected, cleaned and wiped dry in a timely manner. The rotor is the key component to be protected in the centrifuge. Be careful when moving it. Do not collide and avoid injuries. When turning for long periods, do not use it. Apply a layer of polishing wax to protect it. Do not use the centrifuge tube with significant deformation, damage or aging.